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Step 4: Slowly melt the butters and beeswax in a microwave for 30 seconds at a time. The Beeswax will melt with the oils. Stir between each 30-second set. When it is almost melted and there are small chunks left in the container, remove from the microwave and stir with a knife or spoon and the remaining will melt, cooling the mixture at the same time.
Once the agar has hardened, invert the petri dish. Incubate this control plate with the serial test plates. 3.3.5 Preparation of the VRBA medium: On the day of the test, melt the VRBA in an autoclave for 5-10 minutes at 100°C. Place the bottles of melted media in the bead bath. Do not begin testing until the agar has cooled to 55°C. The
For more information, visit http://www.bio-rad.com/yt/3/pGLOThis video demonstrates how to use a hot plate to melt the LB Agar in preparation for pouring pl...
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Apr 22, 2015 · 3. Water – 1/2 Cup 4. Agar – 1 tbspn flakes* 5. Saffron – 4 to 5 strands of good quality saffron 6. Ziploc Bag – 1 7. A Pair of Scissors 8. A water bath (a bowl filled with water, preferably cold) * I used 1.75 agar blocks which when crushed gave me 1 tablespoon flakes.
1 Use a water bath at 50 °C to store bottles of molten agar.. 2 Use plates as soon as possible after pouring. Store in sealed plastic bags to prevent the agar from drying out if they are not going to be used straight away.